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| Title: | Effects of hyperbaric oxygen and platelet derived growth factor on medial collateral ligament fibroblasts. |
| Authors: | Chan, YS
Chen, AS
Yuan, LJ
Lin, SS
Yang, CY
Lee, MS
Ueng, SW |
| Keywords: | Hyperbaric Oxygenation
collagen
collagen fibrils
platelet-derived growth factor-BB
platelet
medial collateral ligament
fibroblast
animal
rabbit |
| Issue Date: | 2007 |
| Publisher: | Undersea and Hyperbaric Medical Society, Inc. |
| Citation: | Undersea Hyperb Med. 2007 May-Jun;34(3):181-90. |
| Abstract: | PURPOSE: This study investigated hyperbaric oxygen (HBO2) and platelet-derived growth factor-BB (PDGF-BB) to determine their combined effects on fibroblasts from rabbit medial collateral ligament (MCL). METHOD: Cells were divided into four groups: (I) Control, (II) HBO2 treatment, (III) PDGF-BB treatment and (IV) HBO2 combined with PDGF-BB treatment. All hyperoxic cells were exposed to 100% O2 at 2.5 atmospheres absolute (ATA) in a hyperbaric chamber for 120 minutes per 48 hours. Measurement of cell growth was based on increase in cell number. Cell cycle modulations were analyzed by fluorescence-activated cell sorter (FACS). Quantity of Type I and Type III collagen was determined by western blotting and image analyzer. RESULTS: Treatment doses of HBO2 alone or PDGF-bb alone dependently increased cell growth. A combination of HBO2 treatment plus PDGF-bb treatment had an additive effect on cell growth in comparison with HBO2 treatment alone or PDGF-bb treatment alone. FACS analysis revealed that HBO2 alone, PDGF-bb alone and PDGF-bb plus HBO2 treatment increase the percentage of cells accumulated in S-phase. Western blotting analysis revealed that Type III collagen content was decreased significantly after HBO2 treatment alone or HBO2 plus PDGF-bb treatment but not in PDGF-bb treatment alone. In contrast, although Type I collagen content was increased after HBO2 treatment, the increase in Type I collagen (increase /original) was not statistically significant. CONCLUSION: HBO2 or HBO2 plus PDGF-bb treatment decreases the Type III collagen/Type I collagen content, which could result in mechanically stronger collagen fibrils. We propose HBO2 therapy as a potentially effective treatment for MCL healing. |
| Description: | Undersea & Hyperbaric Medicine : Journal of the Undersea and Hyperbaric Medical Society, Inc. |
| URI: | http://archive.rubicon-foundation.org/7348 |
| ISSN: | 1066-2936 |
| Appears in Collections: | Undersea and Hyperbaric Medicine Journal
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| 17672174.pdf | | 1625Kb | Adobe PDF | View/Open |
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