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| Title: | DECOMPRESSION SICKNESS RISK CORRELATED WITH ACTIVITY OF H2-METABOLIZING MICROBES INJECTED IN PIGS PRIOR TO DIVES IN H2. |
| Authors: | Fahlman, A
Kayar, SR
Becker, WJ
Lin, WC
Whitman, WB |
| Keywords: | decompression
decompression
dry
hyperbaric
chamber
model |
| Issue Date: | 1999 |
| Abstract: | BACKGROUND: Biochemical decompression, a novel approach for decreasing decompression sickness (DCS) risk by increasing the tissue washout rate of the diluent gas, was tested in an animal model during simulated H2 dives. Logistic regression was used to show that the DCS risk was negatively correlated with the biochemical activity used to decrease the tissue gas burden. METHODS: To increase the washout rate of H2, we used a H2-metabolizing microbe (Methanobrevibacter smithii) that converts H2 to CH4. Pigs (Sus scrofa, 19.4+ 1.3 kg) were either untreated controls (n=10), saline-injected controls (n=10) or injected with M.smithii into the large intestine (n=14; varying total activity 200-2200 ^mol CH4/min). The pigs were placed in a dry hyperbaric chamber and compressed to 24 bar (20.6-22.9 bar H2, 0.3-0.5 bar O2). Chamber gases (O2, N2, He, H2, and CH4) were monitored using gas chromatography throughout the dive. After 3 h, the pigs were decompressed to 11 bar at 0.9 bar/min, and observed for severe symptoms of DCS for 1 h. RESULTS: Pigs with M. smithii had a 46percent lower incidence of DCS compared to controls (6/14 vs. 16/20). Using logistic regression techniques, we shower that the DCS risk rate decreased with increasing total activity of microbes injected (P= 0.015, Log-Likelyhood ration = 5.965, 1 df). CONCLUSION: Reducing the tissue concentration of the inert gas significantly reduced the risk of DCS in a pig model. (NMRDC #61153N MR04101.00D-1103). |
| Description: | Undersea and Hyperbaric Medical Society, Inc. (http://www.uhms.org ) |
| URI: | http://archive.rubicon-foundation.org/787 |
| Appears in Collections: | UHMS Meeting Abstracts
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