|
|
Rubicon Research Repository >
Rubicon Foundation Archive >
Thesis Publications >
Please use this identifier to cite or link to this item:
http://archive.rubicon-foundation.org/4781
|
| Title: | STRESS BIOMARKERS IN A RAT MODEL OF DECOMPRESSION SICKNESS. |
| Authors: | Caviness, J
Montcalm-Smith, E |
| Keywords: | Decompression
Decompression Sickness
Hyperbaric
biomarker
Sprague Dawley rats
animal |
| Issue Date: | 2005 |
| Publisher: | UNIFORMED SERVICES UNIV OF THE HEALTH SCIENCES BETHESDA MD |
| Citation: | ADA439127 |
| Abstract: | BACKGROUND: Stress-induced inflammatory reactions may contribute to decompression sickness (DCS). To date there is no biomarker for DCS. This research used ELISA and PCR to investigate the effects of hyperbaric pressure on genomic/proteomic responses of 6 stress sensitive markers [hemoxygenase-1 (HO-1), Heat shock protein (HSP) 27, HSP 70, early growth response factor (EGR)-1, iNOS, P-selectin, C-reactive protein (CRP) and endothelin-1 (ET-1)MATERIALS AND METHODS: Sprague Dawley rats were exposed to hyperbaric pressure (175 fsw/5.2 ATA) for 60 min, then rapidly (less than 15 sec) decompressed. After surfacing, animals were observed for 30 min for signs of DCS (ambulatory deficit) while walking in a rotating cage (approx.3 m/min). Animals were then euthanized for collection of blood and tissue (brain, liver, lung). Serum levels of CRP and ET-1 were performed by ELISA. PCR was used for of HO-1, HSP 27 and 70, EGR-1, iNOS and P-selecti RESULTS: In animals with DCS, HO-1 and HSP 27 were increased in the brains and HO-1, EGR-1 and iNOS were increased in the lungs. There were no significant changes in the liver; nor were there any differences in levels of HSP 70, ET-1, CRP or P-selecti CONCLUSIONS: These results indicate that DCS, like other stressors, alters gene expression. Moreover, these alterations occur rapidly (less than30 min) after surfacing. Future studies will determine if some of the above markers are specific for DCS or a general non-specific stress response. Since EGR-1 is known to trigger expression of regulators for inflammation, perhaps antagonism of EGR-1 has potential as a treatment for DCS. With increased understanding of underlying biochemical changes that occur with hyperbaric exposure, future research may lead to a clinical test for DCS. |
| Description: | Citation Status: Active; Citation Classification: Unclassified; Title Classification: Unclassified; Report Classification: Unclassified; Identifier Classification: Unclassified; Abstract Classification: Unclassified; Distribution Limitation(s): 01 - APPROVED FOR PUBLIC RELEASE; Information provided by the Department of Defense and the Defense Technical Information Center (http://www.dtic.mil/) is considered public information and may be distributed or copied unless otherwise specified. Use of appropriate byline/photo/image credits is requested. |
| Gov't Doc # : | ADA439127 |
| URI: | http://archive.rubicon-foundation.org/4781 |
| Appears in Collections: | Thesis Publications
|
Files in This Item:
| File |
Description |
Size | Format |
|---|
| CavinessMS.pdf | | 200Kb | Adobe PDF | View/Open |
|
All items in DSpace are protected by copyright, with all rights reserved.
|
