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| Title: | Voltage sensitive dye imaging of transient neuronal assemblies in brain slices under hyperbaric conditions. |
| Authors: | Wlodarczyk, A
McMillan, PF
Greenfield, SA |
| Keywords: | voltage sensitive dye
pressure-reversal
Brain slices
animal
rat |
| Issue Date: | 2008 |
| Publisher: | Undersea and Hyperbaric Medical Society |
| Citation: | Undersea Hyperb Med. 2008 Jan-Feb;35(1):35-40. |
| Abstract: | We have developed a high pressure optical cell to study large-scale transient neuronal coalitions--"assemblies" using voltage sensitive dye (VSD) fluorescence combined with fast CCD imaging of brain slices under hyperbaric conditions. The new cell has been tested at pressures up to P = 150-200 atm, corresponding to the range over which effects such as "pressure-reversal" of anaesthesia have been described previously. Brain slices were maintained in a flow of artificial cerebrospinal fluid (aCSF) and hyperbaric conditions were controlled to within +/- 0.2 atm using a back-pressure regulator placed in the pumping system. Preliminary VSD imaging experiments were carried out on rat hippocampal slices at pressures up to P approximately 50 atm. An electrode placed in the CA3 region was used to stimulate a signal along the Schaffer collateral towards CA1. First results indicate that good VSD data can be obtained that can be analysed to provide a new view on how hydrostatic pressurisation affects the dynamic propagation of neuronal assemblies. |
| Description: | Xth Meeting International High Pressure Biology Group, Undersea & Hyperbaric Medicine : Journal of the Undersea and Hyperbaric Medical Society, Inc. |
| URI: | http://archive.rubicon-foundation.org/8047 |
| ISBN: | 1066-2936 |
| Appears in Collections: | Undersea and Hyperbaric Medicine Journal
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| 18351125.pdf | | 1464Kb | Adobe PDF | View/Open |
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