[abstract]MICROPARTICLE PRODUCTION, NEUTROPHIL ACTIVATION AND INTRAVASCULAR BUBBLES FOLLOWING OPEN-WATER SCUBA DIVING

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[abstract]MICROPARTICLE PRODUCTION, NEUTROPHIL ACTIVATION AND INTRAVASCULAR BUBBLES FOLLOWING OPEN-WATER SCUBA DIVING

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Title: [abstract]MICROPARTICLE PRODUCTION, NEUTROPHIL ACTIVATION AND INTRAVASCULAR BUBBLES FOLLOWING OPEN-WATER SCUBA DIVING
Author: Thom SR; Milovanova TN; Bogush M; Bhopale VM; Yang M.; Bushmann K; Pollock NW; Ljubkovic M; Denoble PJ; Dujic Z
Abstract: Introduction: Decompression stress increases the number of circulating pro-inflammatory microparticles (MPs). Injecting these into naïve mice recapitulates decompression-induced vascular injuries in a mouse model (J Appl Physiol. 2012; 112:204). The goal of this study was to evaluate MPs and neutrophil activation in humans following decompression from open-water scuba dives and determine if changes are related to intravascular bubble formation. Methods: Sixteen male volunteer divers followed a uniform profile of four daily exercising scuba dives to 18 meters of sea water for 47 minutes. Blood was obtained prior to and 80 minutes following the first and fourth dives to evaluate the impact of repetitive diving on MPs and neutrophil activation. Intravascular bubbles were quantified by transthoracic echocardiography at 20-minute intervals for two hours post-dive. Results: MPs increased by 2.4-fold after each dive (no significant difference between Dive 1 and Dive 4). Neutrophil activation occurred after each dive as assessed by surface expression of myeloperoxidase and the CD18 component of ?2 integrins, and there were increased platelet membrane interactions assessed by presence of the platelet-derived CD41 protein on the neutrophil surface. Intravascular bubbles were detected in all divers. Surprisingly, significant inverse correlations were found among post-dive bubble scores and MPs, which may relate to having only acquired an 80-minute blood sample. There were significant positive correlations between MPs and platelet-neutrophil interactions after the first dive and between platelet-neutrophil interactions and neutrophil activation (documented as elevation in ?2 integrin expression) after the fourth dive. Conclusion: MPs- and neutrophil-related events in humans are consistent with findings in an animal decompression model. Whether there are causal relationships among bubbles, MPs, platelet-neutrophil interactions and neutrophil activation remains obscure and requires additional study. Financial Disclosure: This activity was supported by grants from the Office of Naval Research, Divers Alert Network (DAN), Unity through Knowledge Fund and Croatian Ministry of Science, Education and Sports.
Description: Undersea and Hyperbaric Medicine : Journal of the Undersea and Hyperbaric Medical Society, Inc.
URI: http://archive.rubicon-foundation.org/10442
Date: 2012

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  • UHMS Meeting Abstracts
    This is a collection of the published abstracts from the Undersea and Hyperbaric Medical Society (UHMS) annual meetings.

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