[abstract]ENHANCEMENT OF INJURED SKELETAL MUSCLE RECOVERY BY HYPERBARIC OXYGEN TREATMENT

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[abstract]ENHANCEMENT OF INJURED SKELETAL MUSCLE RECOVERY BY HYPERBARIC OXYGEN TREATMENT

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Title: [abstract]ENHANCEMENT OF INJURED SKELETAL MUSCLE RECOVERY BY HYPERBARIC OXYGEN TREATMENT
Author: Horie M; Enomoto M; Shimoda M; Okawa A; Miyakawa S; Yagishita K
Abstract: OBJECTIVE: Recently, hyperbaric oxygen (HBO2)treatment is widely applied for sports-related soft tissue injuries, including muscle injury, for the purpose of rapid recovery. However, the effects and mechanisms of hyperbaric-hyperoxia exposure during HBO2 treatment on muscle injury remain obscure. In this study, we investigated the effects of hyperbaric-hyperoxia exposure on skeletal muscle regeneration following muscle injury. METHODS: Muscle injury was induced by injecting cardiotoxin (CTX) into rat tibialis anterior muscles. The animals were divided into four groups: non-treatment (NT), hyperbaric oxygen treatment (HBO2), normobaric oxygen treatment (NBO2) and hyperbaric air treatment (HBA). Animals in the HBO2 group were placed in a hyperbaric experimental chamber in which 100 percent oxygen was administered at 2.5 atmospheres absolute (atm abs) pressure. The NBO2 group underwent 100 percent oxygen administration at 1 atm abs pressure. The HBA group underwent air administration at 2.5 atm abs pressure. All treatment time were two hours; treatments were started the day after the CTX injections and continued for two weeks (five days a week). We then measured the maximum force capacity of the regenerating muscle fibers and performed histological analyses and quantitative RT-PCR for muscle regulatory factors. RESULTS: The cross-sectional areas and maximum force capacities of the regenerating muscle fibers were significantly higher in the HBO2 group compared to the other groups. MyoD, Myogenin and IGF-1 mRNA levels were significantly increased in the HBO2 group at Day 3 or Day 5 after injury. The number of MyoD-positive nuclei was significantly greater in the HBO2 group compared to the other groups at day 5 after injury. CONCLUSION: In this study, we demonstrated that hyperbaric-hyperoxia exposure promoted satellite cell differentiation and accelerated myofiber maturation in injured muscle. These results indicate that HBO2 treatment accelerates healing and functional recovery after muscle injury.
Description: Undersea and Hyperbaric Medicine : Journal of the Undersea and Hyperbaric Medical Society, Inc.
URI: http://archive.rubicon-foundation.org/10665
Date: 2013

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  • UHMS Meeting Abstracts
    This is a collection of the published abstracts from the Undersea and Hyperbaric Medical Society (UHMS) annual meetings.

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