[abstract] NEUROPROTECTIVE EFFECTS OF HYPERBARIC OXYGEN TREATMENT IN EXPERIMENTAL FOCAL CEREBRAL ISCHEMIA ARE ASSOCIATED WITH REDUCED BRAIN LEUKOCYTE MYELOPEROXIDASE ACTIVITY

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[abstract] NEUROPROTECTIVE EFFECTS OF HYPERBARIC OXYGEN TREATMENT IN EXPERIMENTAL FOCAL CEREBRAL ISCHEMIA ARE ASSOCIATED WITH REDUCED BRAIN LEUKOCYTE MYELOPEROXIDASE ACTIVITY

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Title: [abstract] NEUROPROTECTIVE EFFECTS OF HYPERBARIC OXYGEN TREATMENT IN EXPERIMENTAL FOCAL CEREBRAL ISCHEMIA ARE ASSOCIATED WITH REDUCED BRAIN LEUKOCYTE MYELOPEROXIDASE ACTIVITY
Author: Rosenthal, RE; Miljkovic-Lolic, M; Silbergleit, R; Fiskum, G
Abstract: OBJECTIVE: Hyperbaric oxygen (HBO) reduces cerebral infarct size after middle cerebral artery occlusion (MCAO) in rats through an unknown mechanism. In other forms of injury, cellular protection with HBO is associated with diminished infiltration of polymorphonuclear neutrophils (PMN). We hypothesized that HBO given prior to or after MCAO reduces PMN infiltration into the brain, and that decreased PMN infiltration is associated with improved functional and anatomic outcome. METHODS: 40 rats underwent MCAO and were randomized to pretreatment with HBO (3 ATA) immediately prior to (n=13), or post treatment immediately after surgery (n=12), or to control (air 1 ATA) (n=15). 5 rats underwent sham surgery. Neurologic outcome was measured at 24 hours in all animals. Brain myeloperoxidase (MPO) activity (n=22) and infarct volume (n=23) were determined. RESULTS: MPO activity was significantly higher in controls (mean 0.28, 95percent CI 0.17-0.38) than in the HBO pretreatment group (0.12, 0.08-0.16), HBO post treatment group (0.16, 0.13-0.19), and the sham group (0.02, -0.02-0.05). HBO treated animals also had better neurologic outcomes (pretreatment 1.5, 0.9-2.1, post treatment 2.6, 2.0-3.2) and smaller infarcts (pretreatment 27percent, 18-37percent, post treatment 28percent, 19-37percent) than controls (neurologic outcome 3.7, 3.1-4.4, infarct volume 39percent, 30-48percent). Neurologic outcomes correlate better with MPO activity (R2=0.75) than with infarct volume (R2=0.25). CONCLUSIONS: These data confirm the neuroprotective effects of HBO in cerebral ischemia and suggest that the mechanism of this action may involve inhibition of PMN infiltration in the injured brain. Hyperbaric oxygenation, stroke, myeloperoxidase, leukocyte
Description: Undersea and Hyperbaric Medical Society, Inc. (http://www.uhms.org )
URI: http://archive.rubicon-foundation.org/1330
Date: 2003

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  • UHMS Meeting Abstracts
    This is a collection of the published abstracts from the Undersea and Hyperbaric Medical Society (UHMS) annual meetings.

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