[abstract] LONGTERM SURVIVAL OF POTENTIALLY LETHAL HEMORRHAGIC SHOCK AFTER TREATMENT WITH INTRAVASCULAR MICROBUBBLES

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[abstract] LONGTERM SURVIVAL OF POTENTIALLY LETHAL HEMORRHAGIC SHOCK AFTER TREATMENT WITH INTRAVASCULAR MICROBUBBLES

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Title: [abstract] LONGTERM SURVIVAL OF POTENTIALLY LETHAL HEMORRHAGIC SHOCK AFTER TREATMENT WITH INTRAVASCULAR MICROBUBBLES
Author: Lundgren, CEG; Bergoe, GW; Tyssebotn, I
Abstract: INTRODUCTION: Dodecafluoropentane (DDFP) has a boiling point of 29 C. Injected intravenously and warmed to 37 C a 2percent DDFP emulsion (DDFPe) forms subcapillary sized microbubbles that transport oxygen from the lungs to the tissues. We have earlier shown that this preparation sustains life in erythrocyte depleted normovolemic rats and supports short-term (hours) survival of hemorrhagic shock in anesthetized pigs. The present study describes experiments in which severely bled pigs treated with DDFPe were allowed to wake up from anesthesia and observed for 14 days. METHODS: Spontaneously air breathing anesthetized pigs were instrumented for physiological monitoring and bled greater than 35 ml/kg (" 50percent of blood volume) in about 60 min. Subsequently, five pigs received ~ 0.4 ml DDFPe/kg i.v. while five controls were injected with vehicle after which they all were monitored for up to 4.5 hrs. Survivors then received ~ 60percent of shed blood volume in the form of Hespan, were taken out of anesthesia and returned to the pen for 11-14 days of observation followed by final physiological monitoring, euthanasia and autopsy. RESULTS: Of the controls 4/5 died in between 3-5 hrs while 5/5 DDFPe treated animals all survived and, at comparable times, had significantly better blood pressure, diuresis, acid-base, electrolyte status and oxygen tensions in mixed venous blood and muscle tissue. During the long-term observation in the pen there were significant differences in favor of the DDFPe-treated animals compared to the surviving control, in terms of' weight gain, kidney function and, after sacrifice, in autopsy findings and histology of numerous organs. CONCLUSIONS: These results (if applicable to humans) strongly suggest that hypoxia is a crucial element in the pathophysiology of hemorrhagic shock and that DDFPe treatment with extremely small doses (supplying 0.008 ml DDFP/kg) may extend the "Golden Hour " several fold before full-resource treatment must be given.
Description: Undersea and Hyperbaric Medical Society, Inc. (http://www.uhms.org )
URI: http://archive.rubicon-foundation.org/1485
Date: 2004

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  • UHMS Meeting Abstracts
    This is a collection of the published abstracts from the Undersea and Hyperbaric Medical Society (UHMS) annual meetings.

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