Sensitivity of cultured mammalian cells to oxidative stress: adaptation to repeated exposures of hyperbaric oxygen

Rubicon Research Repository/Manakin Repository

Sensitivity of cultured mammalian cells to oxidative stress: adaptation to repeated exposures of hyperbaric oxygen

Show full item record


Title: Sensitivity of cultured mammalian cells to oxidative stress: adaptation to repeated exposures of hyperbaric oxygen
Author: Messier, AA; Fisher, HW
Abstract: Four established cell lines (mouse neuroblastoma, N2A; Chinese hamster lung, V79; Chinese hamster ovary, CHO; and rabbit kidney, RK13) were made O2-tolerant by repetitive exposure to hyperbaric oxygen (HBO). The cultures were exposed to 100% O2 at pressures ranging from 6 to 10 ATA for time periods up to 3 h, and the surviving cells were regrown to monolayer confluency and reexposed; by the end of three cycles of treatment these cells were tolerant to exposures of 10 ATA O2 for greater than 5 h. The development of O2 tolerance was measured by enzyme and morphologic indices. Results showed that all of the cell lines tested could be made O2 resistant. However, qualitative differences were found. RK13 cells were more resistant to HBO than the other cell types tested. The technique provides a powerful adjunct to current methods that study the effects of oxidative stress in mammalian cells. The ability to generate O2-resistant cells in only 3-4 wk provides a considerable time savings over published efforts (12-20 mo.). In addition, rapid screening of various cell lines may lead to discovery of O2-resistant cell types which will provide insight into the factors inherent in the development of oxygen tolerance.
Description: Undersea and Hyperbaric Medical Society, Inc. (http://www.uhms.org )
URI: PMID: 2168598
http://archive.rubicon-foundation.org/2548
Date: 1990

Files in this item

Files Size Format View
2168598.pdf 2.632Mb PDF View/Open

This item appears in the following Collection(s)

  • Undersea Biomedical Research Journal
    The Undersea Baromedical Research journal was published by the Undersea Medical Society, Inc. (now the Undersea and Hyperbaric Medical Society) quarterly from 1974 to 1992 when the name changed to the Undersea and Hyperbaric Medicine Journal.

Show full item record

Browse

My Account