Method for separation of cells and suspending medium at high pressure

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Method for separation of cells and suspending medium at high pressure

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Title: Method for separation of cells and suspending medium at high pressure
Author: Goldinger, JM; Kang, BS; Morin, RA; Paganelli, CV; Hong, SK
Abstract: We describe a pressure chamber designed for measuring fluxes and equilibrium distributions of substances between cells in suspension and the extracellular medium at pressures up to 400 ATA. The pressure chamber contains a filtration apparatus capable of taking four separate samples of cell-free medium from a cell suspension at timed intervals, without necessity of decompression. In addition, a high pressure injection system makes possible the addition to a cell suspension of precise quantities of agents such as ionophores or metabolic inhibitors, for observations of their effects on cell functions at pressure. The chamber of filtration apparatus functioned successfully in preliminary experiments designed to measure the equilibrium distribution of 36Cl- in human erythrocytes at high pressure. *Atmospheric Pressure Culture Media *Cytological Techniques Erythrocytes/physiology Human In Vitro
Description: Undersea and Hyperbaric Medical Society, Inc. (http://www.uhms.org )
URI: PMID: 7013220
http://archive.rubicon-foundation.org/2901
Date: 1981

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  • Undersea Biomedical Research Journal
    The Undersea Baromedical Research journal was published by the Undersea Medical Society, Inc. (now the Undersea and Hyperbaric Medical Society) quarterly from 1974 to 1992 when the name changed to the Undersea and Hyperbaric Medicine Journal.

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