A versatile chamber for microphysiologic studies with gas mixtures under high pressure

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A versatile chamber for microphysiologic studies with gas mixtures under high pressure

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Title: A versatile chamber for microphysiologic studies with gas mixtures under high pressure
Author: Fagni, L; Hugon, M; Folco, A; Imbert, G
Abstract: A pressure chamber, designed for microelectrode recordings in isolated tissues or organs an capable of withstanding pressures up to 200 bar, is described. The versatility of the vessel allows a wide variety of experimental configurations and several types of studies. It features a complete access, easy visibility, interchangeable tissue bath and micromanipulator modules, as continuous perfusion and temperature control of the preparation under pressure. The ability to move micropipettes in 1-micron steps allows single unit recording on a variety of in vitro preparations at normal or elevated gas pressures. Successful physiologic tests, using the hippocampal slice preparation, are described. The results testify to the reliability of the system and to the usefulness of this in vitro model to study the gas pressure effects on isolated networks of mammalian CNS. *Air Pressure Animals *Atmosphere Exposure Chambers *Atmospheric Pressure Central Nervous System/*physiology Evoked Potentials Gases Helium Rats Rats, Inbred Strains Support, Non-U.S. Gov't
Description: Undersea and Hyperbaric Medical Society, Inc. (http://www.uhms.org )
URI: PMID: 3576846
http://archive.rubicon-foundation.org/3088
Date: 1987

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  • Undersea Biomedical Research Journal
    The Undersea Baromedical Research journal was published by the Undersea Medical Society, Inc. (now the Undersea and Hyperbaric Medical Society) quarterly from 1974 to 1992 when the name changed to the Undersea and Hyperbaric Medicine Journal.

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