Methods to Obtain Blood Samples Periodically During Exercise Research Studies While Subjects are Immersed in Water or Otherwise Inaccessible.

Abstract

We describe a method to obtain periodic blood samples during exercise studies by use of an indwelling intravenous catheter. A step-by-step process for establishing the intravenous line and obtaining samples is also described. Steps we have found especially useful to facilitate the process and to avoid complications are presented in more detail. A list of supplies required for the process is included. With these methods, we have been able to obtain samples from subjects performing heavy exercise on cycle ergometers and from other subjects who were immersed for up to three hours in water at 18 deg C and working on an underwater ergometer. Hemorrhage, infection or other complications did not occur. The only failure has been occasional inability to obtain a blood sample because blood flow was inadequate.... Exercise, Blood samples.