Effect of oxygen on phagocytic and adherence functions in mouse spleen macrophages.

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Effect of oxygen on phagocytic and adherence functions in mouse spleen macrophages.

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Title: Effect of oxygen on phagocytic and adherence functions in mouse spleen macrophages.
Author: Mehm, WJ; Pimsler, M
Abstract: The effects of varied of varied oxygen partial pressures were tested on the phagocytic and adherence functions of mouse spleen macrophages. The evaluation was accomplished in vitro using standard cytologic and microscopic techniques. Macrophages were harvested from mouse spleens, placed in culture on glass coverslips and fed latex heads, and allowed to feed for varying time intervals. During the feeding period, cultures were exposed to one of several oxygen partial pressures via an environment chamber or hyperbaric chamber. After treatment, macrophages were fixed and stained and cell counts accomplished to record the total number of macrophages adhered and the total number of bead-positive macrophages present in a sample of 20 fields/slide. Our data indicate that short-term exposure of mouse spleen macrophages to relatively high oxygen tensions does not affect either their rate of phagocytosis or adherence to glass slides. These findings suggest that it is not the effect of oxygen on macrophage adherence or phagocytic activity during wound debridement that is responsible for enhanced wound healing in response to hyperbaric oxygen therapy.
Description: Journal of Hyperbaric Medicine : Journal of the Undersea and Hyperbaric Medical Society, Inc.
URI: http://archive.rubicon-foundation.org/4317
Date: 1986

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