[abstract] IN VITRO EFFECTS OF OXYGEN ON CELL TYPES PARTICIPATING IN THE WOUND HEALING PROCESS.

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[abstract] IN VITRO EFFECTS OF OXYGEN ON CELL TYPES PARTICIPATING IN THE WOUND HEALING PROCESS.

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Title: [abstract] IN VITRO EFFECTS OF OXYGEN ON CELL TYPES PARTICIPATING IN THE WOUND HEALING PROCESS.
Author: Mehm, WJ; Pimsler, M; Becker, RL; Lissner, CR
Abstract: In this study, the biological effects of several oxygen tensions were investigated in cocultures of macrophages and fibroblasts harvested from the spleens of mice. Evaluations were accomplished in vitro using standard cytological, electron microscopic, and biochemical techniques. Cells were harvested from spleens, placed in culture on glass coverslips and exposed in an environment chamber or hyperbaric chamber to one of several oxygen partial pressures for varying time intervals. After exposure, cell cultures were examined by the following techniques to evaluate oxygen effects. Macrophage adherence and rate of phagocytosis were quantified via total macrophage cell counts per coverslip and number of bead positive macrophages (cells having consumed five or more latex beads). A microscope morphometer was used to determine rates of fibroblast proliferation directly from the coverslips over a nine day growth period. The scanning electron microscope was used to evaluate cell morphol6gy and surface contour. Additionally, ATP and collagen production were evaluated via luminometry and spectrophotometric analysis of hydroxyproline, respectively. Our data suggest that short-term exposure of cells in culture to relatively high oxygen tensions does not affect either phagocytosis of latex beads or glass adherence by mature stromal macrophages from mouse spleens. Preliminary results from scanning electron microscopy indicate macrophage morphology and surface contour to be unaffected by varying oxygen tensions.
Description: Abstract of the Undersea and Hyperbaric Medical Society, Inc. Annual Scientific Meeting held May 26-30, 1987. The Hyatt Regency Hotel, Baltimore, Maryland (http://www.uhms.org)
URI: http://archive.rubicon-foundation.org/5599
Date: 1987

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  • UHMS Meeting Abstracts
    This is a collection of the published abstracts from the Undersea and Hyperbaric Medical Society (UHMS) annual meetings.

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