[abstract] EFFECT OF OXYGEN TENSION ON MACROPHAGE MIGRATION IN A COMPLEMENT C5a GRADIENT.

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[abstract] EFFECT OF OXYGEN TENSION ON MACROPHAGE MIGRATION IN A COMPLEMENT C5a GRADIENT.

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Title: [abstract] EFFECT OF OXYGEN TENSION ON MACROPHAGE MIGRATION IN A COMPLEMENT C5a GRADIENT.
Author: Anderson, LH; Mehm, WJ
Abstract: BACKGROUND: Macrophages have been reported to be one of the first cells to migrate to the hypoxic center of a wound. This study was designed to determine if the oxygen tension during migration effects the chemotactic response of macrophages within a complement C5a gradient. This study will predict the effects of hyperbaric therapy on the chemotactic response of macrophages. METHODS: Macrophages (RAW 264.7) were grown under normal culture conditions. Chemotactic response to complement C5a was assayed at 1.95, 5.6 (tissue normoxia), 12.2, 22.0 and 104.1 kPa oxygen as follows: 10-s M complement C5a in media was placed in the bottom wells of the assay chamber and covered with a polycarbonate membrane. Macrophages (1 x 106 cells/ml in O2 tension-adjusted medium) were placed in the top wells of the chamber. The cells were incubated 2 hr at 3JOC at the desired oxygen tension. The oxygen tension remained constant during incubation, while the complement C5a gradient was formed by diffusion. After incubation, the membrane was fixed and stained. The cells which migrated through the membrane were counted. RESULTS: When macrophage chemotactic response was assayed at 5.6 kPa, it was significantly different from the response at any other oxygen tension tested. A significant increase (p < 0.05) was seen when the assay oxygen tension was 12.2 kPa. When the chemotactic response was assayed at 1.95, 22.0 or 104.1 kPa, it was significantly lower (p < 0.05) than the response at 5.6 kPa. CONCLUSION: These preliminary data indicate that there is a range of oxygen tensions at which the macrophage migratory function is optimal. This range apparently spans from normal tissue oxygen tension (5.6 kPa) to twice that oxygen tension (12.2 kPa). Increasing or decreasing the oxygen tension beyond that impairs the migratory response.
Description: Abstract of the Undersea and Hyperbaric Medical Society, Inc. Annual Scientific Meeting held June 22-26, 1994. Westin Hotel, Denver, Colorado (http://www.uhms.org)
URI: http://archive.rubicon-foundation.org/5690
Date: 1994

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  • UHMS Meeting Abstracts
    This is a collection of the published abstracts from the Undersea and Hyperbaric Medical Society (UHMS) annual meetings.

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