[abstract] STABILIZED MICROBUBBLES AS A BLOOD SUBSTITUTE

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[abstract] STABILIZED MICROBUBBLES AS A BLOOD SUBSTITUTE

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Title: [abstract] STABILIZED MICROBUBBLES AS A BLOOD SUBSTITUTE
Author: Tyssebotn, I; Lundgren, CEG; Bergoe, GW; Van Liew, HD; Goldinger, J
Abstract: Van Liew and Burkard (J.Appl. Physiol. 81:500-508, 1996) predicted theoretically that stabilized microbubbles might be capable of supporting exchange of physiological gases between lungs and tissues. This hypothesis was tested by hemoglobin depletion in anesthetized rats given i.v. infusion of dodecafluoreopentane (DDFP) emulsion (EchoGen, SONUS Pharmaceuticals, Inc., Seattle, WA) which forms stabilized microbubbles in vivo. The rats (n=7) were bled to lower hemoglobin while circulatory volume was maintained by simultaneous infusion of isomotic albumin - Ringer's solution. A control group of rats (n=7) was similarly bled, but recieved on ly albumin-Ringer's solution and the emulsion vehicle. Invasive recordings of arterial and central venous pressures were performed and local O2 and CO2 tensions in skeletal muscle were recorded with surface and needle electrodes. Breathing was spontaneous and both groups inhaled 70percent of O2 in N2. Within 80 min. after the start of hemodilution, the control animals experienced irreversible loss of blood pressure and severly reduced muscle PO2, at an average hemoglobin concentration of 2.8 g/100ml. On the other hand, the EchoGen-treated rats survived for 4-5 hours haing spent 2-3 hours with a hemoglobin concentration averaging 1.3 g/100ml. Arterial and venous pressures, heart rate, respiratory rate, tissue PO2 and PCO2 levels, and urine production remained normal until euthanasia. We conclude that the DDFP micropbubbles in the circulation can sustain physiological gas exchange in the abscence of oxygen-enriched gas mixture. This novel method may allow the development of a clinically useful blood substitute (Van Liew, H. Burkard, M. Lundgren, C. Tyssebotn, I US patent allowed, number pending). Acknowledgement: This project was supported in part by SONUS Pharmaceuticals, Inc., Seattle, WA.
Description: Undersea and Hyperbaric Medical Society, Inc. (http://www.uhms.org )
URI: http://archive.rubicon-foundation.org/845
Date: 1999

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  • UHMS Meeting Abstracts
    This is a collection of the published abstracts from the Undersea and Hyperbaric Medical Society (UHMS) annual meetings.

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