[abstract] DODECAFLUOROPENTANE (DDFP) STABILIZED MICROBUBBLES SUPPORT LIFE WITHOUT BLOOD IN AWAKE RATS

Rubicon Research Repository/Manakin Repository

[abstract] DODECAFLUOROPENTANE (DDFP) STABILIZED MICROBUBBLES SUPPORT LIFE WITHOUT BLOOD IN AWAKE RATS

Show full item record


Title: [abstract] DODECAFLUOROPENTANE (DDFP) STABILIZED MICROBUBBLES SUPPORT LIFE WITHOUT BLOOD IN AWAKE RATS
Author: Tyssebotn, I; Bergoe, GW; Goldinger, J; Lundgren, CEG
Abstract: In recent experiments we have shown that stabilized DDFP microbubbles (EchoGen, SONUS Pharmaceuticals, Inc.) can transport O2 and CO2 in sufficient amounts to allow anesthetized rats with less than 10percent of their normal hemoglobin (Hb) to exibit normal circulatory parameters and live for more than 2 hours (intentional termination). In the present study, the ability of such bubbles to transport O2 anc CO2 was tested in awake rats, spontaneously breathing 100percent O2. During a brief anesthesia, the rats were instrumented to provide arterial pressure (AP) and heart rate (HR); through a carotid artery, blood was removed at the same rate as 7percent albumin in Ringer's solution was infused into a jugular vein. Isovolumetric hemodilution was done identically in two series of experiments until the Hb was 50percent of the initial concentration whereafter the test group (7 rats) received a slow infusion of DDFP emulsion in Ringer's solution (total DDFP dose: 8-10 ul) and the control group (7 rats) received the EchoGen vehicle in Ringer's solution. The control rats could not be brought out of the anesthesia, exibited an irreversible loss of blood pressure, and died at Hb concentration of 2.8 g/100ml. By contrast, all DDFP-treated rats, having an average Hb concentration of 1.4 g/100ml, gradually woke up in the O2 filled cage. They started to walk around, ate, drank, and groomed while exibiting normal AP and HR. After 2 hours, they were retransfused with their shed blood until the Hb concentration was above 8 g/100ml (the following day it had increased to 11-12 g/100ml). The AP and HR were normal in daily checks during the next 21 days. The rats appeared and moved normally, and had a normal weight gain. When the rats were sacrificed after 21 days, no macroscopic or microscopic changes were detected in specimens from the brain, heart, lungs, kidney, spleen, liver, and testis. References: I Tyssebotn, C Lundgren, G Bergoe, H Van Liew, J Goldinger. Stabilized microbubbles as a blood substitute and hemoglobin complement. Abstract in this publication. Acknowledgements: Examination of histological specimens by Dr. P. Nickerson is gratefully acknowledged. This project was supported in part by SONUS Pharmaceuticals, Inc., Seattle, WA.
Description: Undersea and Hyperbaric Medical Society, Inc. (http://www.uhms.org )
URI: http://archive.rubicon-foundation.org/846
Date: 1999

Files in this item

Files Size Format View
abstract.txt 259bytes Text file View/Open

This item appears in the following Collection(s)

  • UHMS Meeting Abstracts
    This is a collection of the published abstracts from the Undersea and Hyperbaric Medical Society (UHMS) annual meetings.

Show full item record

Browse

My Account