[abstract] VOLUME-STABILIZED INTRAVASCULAR MICROBUBBLES PROVIDE VITAL OXYGENATION IN HEMOGLOBIN DEPLETED PIGS DURING "AIR BREATHING"

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[abstract] VOLUME-STABILIZED INTRAVASCULAR MICROBUBBLES PROVIDE VITAL OXYGENATION IN HEMOGLOBIN DEPLETED PIGS DURING "AIR BREATHING"

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Title: [abstract] VOLUME-STABILIZED INTRAVASCULAR MICROBUBBLES PROVIDE VITAL OXYGENATION IN HEMOGLOBIN DEPLETED PIGS DURING "AIR BREATHING"
Author: Tyssebotn, I; Bergoe, GW; Lundgren, CEG
Abstract: Intravascular microbubbles generated by i.v. infusion of a dodecafluoropentane emulsion (DDFPe) can transport physiologically significant amounts of oxygen. Thus, rats rendered anemic by bleeding and plasma replacement survived when treated with DDFPe and oxygen, while oxygen breathing controls died (1). The present study explores the question whether DDFPe treatment can similarly sustain life during air breathing in anesthetized pigs. Pigs were bled while given volume replacement with 6percent dextran in lactated Ringer's solution. In all animals, measurement of PaO2 was performed every 15 min. If needed, artificial ventilation and/or oxygen admixture (never greater than than 5percent) to the inspired air was given to maintain PaO2 in the normoxic range of 90-110 mm Hg. A group of control animals (n=6) received emulsion blank in addition to the plasma expander. They died at a mean hemoglobin level of 2.75 g/100 ml after a marked drop in systolic blood pressure which began when the hemoglobin concentration was 5 g/100 ml. The experimental animals received 0.7 ml DDFPe/kg body weight in an i.v. infusion lasting for ~190 min of the 260 min long exsanguination period. These animals survived for more than one hour at hemoglobin levels averaging 2.04 g/100 ml. They retained a normal blood pressure and PaCO2 throughout the experiments. We conclude that perfluorocarbon stabilized microbubbles hold promise as an erythrocyte substitute in situations, such as combat casualty care, even when oxygen is not readily available. 1. Tyssebotn I, Bergoe G, Goldinger J, and Lundgren C. Dodecafluoropentane (DDFP) stabilized microbubbles support life without blood in awake rats. Undersea Hyperbaric Med 26: abstract 97, 1999. Acknowledgment: This study was supported by US Army Medical Research Acquisition Activity Grant Volume-Stabilized Intravascular Microbubbles for Circulatory Transport of Oxygen and Carbon Dioxide: A Field-Usable Concept (ID: DAMD170010514) and by Sonus Pharmaceuticals, Inc.
Description: Undersea and Hyperbaric Medical Society, Inc. (http://www.uhms.org )
URI: http://archive.rubicon-foundation.org/899
Date: 2001

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  • UHMS Meeting Abstracts
    This is a collection of the published abstracts from the Undersea and Hyperbaric Medical Society (UHMS) annual meetings.

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